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1. Algunas propiedades
fisicoquímicas del bacteriófago Mu-1.
J. Martuscelli, F. Bolívar, E. Ramírez y L.
Cañedo
Bol. Estud. Med. Biol. IIBM-UNAM 28: 33-40, (1973).
2. Transformation of Escherichia coli K -12 by linear
DNA from Salmonella typhi.
F. Sánchez, F. Bolívar and J. Martuscelli
Microb. Genet. Bull 38: 13-14, (1975).
3. Supresión fenotípica de mutaciones termosensibles
a la iniciación de síntesis de DNA por la
integración de un factor de resistencia a antibióticos.
F. Bolívar, M.E. Zetina y J. Martuscelli
Bol. Est. Med. Biol. IIBM-UNAM 28: 403-409, (1975).
4. Construction and characterization of cloning vehicles.
R.L. Rodríguez, F. Bolívar, H.M. Goodman,
H.W. Boyer and M. Betlach
In: Molecular Mechanisms in the Control of Gene Expression.
Fox and Rutter (eds.), Academic Press, Inc., New York,
pp. 471-477, (1976).
5. Clonación de ácido desoxirribunucléico
de Neurospora crassa y Escherichia coli, utilizando
tres enzimas de restricción y un vehículo
molecular con sitios específicos para cada una de
ellas.
F. Bolívar, A. Covarrubias y R. Rodríguez
Revista de la Sociedad Química de México 20:
260-264, (1976).
6. Biochemical and genetic characterization of a carbamyl
phosphate synthetase mutant of Escherichia coli K
-12.
F. Bolívar, M. Galván and J. Martuscelli
Journal of General Microbiology 94: 142-148 (1976).
7. Transformation of Escherichia coli by chromosomal
deoxyribonucleic acid from Salmonella typhi.
F. Sánchez, F. Bolívar, and J. Martuscelli
J. Bacteriology 125: 747-749, (1976).
8. Construction and characterization of new cloning vehicles
I Ampicillin resistance derivatives of the plasmid pMB9.
F. Bolívar, R.L. Rodríguez, M. Betlach and
H.W. Boyer
Gene 2: 75-93, (1977).
9. The construction of molecular cloning vehicles.
H.W. Boyer, M. Betlach, F. Bolívar, R.L. Rodríguez,
H.L. Heyneker, J. Shine and H.M. Goodman
In: Recombinant Molecules: Impact on Science and Society
Bers and Banett (eds.), Raven Press, N.Y. pp. 9-20, (1977).
10. Construction and characterization of new cloning vehicles.
II A multipurpose cloning system.
F. Bolívar, R.L. Rodríguez, P.J. Greene, M.C.
Betlach, H.L. Heyneker, H.W. Boyer, J.H. Crosa and S. Falkow
Gene 2: 95-113, (1977).
11. Characterization of tetracycline and ampicillin resistant
plasmid cloning vehicles.
R.L. Rodríguez, R. Tait, J. Shine, F. Bolívar,
H. Heyneker, M. Betlach and H.W. Boyer
In: Molecular Cloning in Recombinant DNA
Miami Winter Symposia 13: 73-78, (1977).
12. Origin of replication of pBR345 plasmid DNA.
F. Bolívar, M.C. Betlach, H.L. Heyneker, J. Shine,
R. L. Rodríguez, and H.W. Boyer
Proc. Natl. Acad. Sci. USA 74: 5265-5269, (1977).
13. The circular restriction map of pBR313.
F. Bolívar, R.L. Rodríguez, M.C. Betlach and
H.W. Boyer
In: DNA Insertion Elements and Episomes
A.I. Bukhari, J.A. Shapiro and S.L. Adhya (eds.), Cold Spring
Harbor Laboratory, USA pp. 684-685, (1977).
14. The circular restriction map of pBR322.
F. Bolívar, R.L.Rodríguez, P.J. Greene, M.C.
Betlach, J.L. Heyneker, H.W. Boyer, J.H. Crosa and S. Falkow
In: DNA Insertion Elements, Plasmids and S. Episomes
A. Bukhari, J.A. Shapiro and S.L. Adhya (eds.) Cold Spring
Harbor Laboratory, USA pp. 686-687, (1977).
15. Expression in Escherichia coli of a chemically
synthesized gene for the hormone somatostatin.
K. Itakura, T. Hiroshi, R. Crea, A.D. Riggs, H.L. Heyneker,
F. Bolívar and H.W. Boyer
Science 198: 1056-1063, (1977).
16. Tetracycline resistance confered by pSC101, pMB9, and
their derivatives.
R. Tait, H.L. Heyneker, R.L. Rodríguez, F. Bolívar,
A. Covarrubias, M. Betlach and H. W. Boyer, Microbiology
In: Proceedings of the Second ASM Conference on Extrachromosomal
Elements. Jackson, Wyoming, USA pp. 174-176, (1978).
17. A general method for the purification of restriction
enzymes.
P.J. Greene, H.L. Heyneker, F. Bolívar, R.L. Rodríguez,
M.C. Betlach, A. Covarrubias, K. Backman, D.J. Russel, R.
Tait and H.W. Boyer
Nucleic Acid Research 5: 2373-2380, (1978).
18. Molecular cloning vectors: derivatives of plasmid pBR322.
F. Bolívar
In: Genetic Engineering
Boyer and Nicosia (eds.), Elsevier, North Holland, pp. 59-64,
(1978).
19. Construction and characterization of new cloning vehicles.
III Derivatives of plasmid pBR322 carrying unique EcoRI
sites for selection of EcoRI generated recombinant
DNA molecules.
F. Bolívar
Gene 4: 121-136, (1978).
20. Expression in Escherichia coli of chemically
synthesized genes for human insulin.
D.V. Goeddel, D.G. Kleid, F. Bolívar, H.L. Heyneker,
D.G. Yansura, R. Crea, T. Hirose, A. Kraszewski, K. Itakura
and A.D. Riggs
Proc. Natl. Acad. Sci. USA 76: 106-110, (1979).
21. Molecular cloning vectors derived from the ColEI type
plasmid pMB1.
F. Bolívar
Life Science 25: 807-818, (1979).
22. Characterization of wild-type and mutant promoters of
the tetracycline resistance gene in pBR313.
R.L. Rodríguez, R.W. West, H.L. Heyneker, F. Bolívar
and H.W. Boyer
Nucleic Acid Research 6: 3267-3287, (1979).
23. Plasmids of Escherichia coli as cloning vectors.
F. Bolívar and K. Backman
Recombinant DNA in: Methods in Enzimology, vol. 68 R.Wu
(ed.) Academic Press, New York, pp. 245-267, (1979).
24. ColEI hybrid plasmids containing Escherichia coli
genes involved in the biosynthesis of glutamate and glutamine.
A.A. Covarrubias, R. Sánchez-Pescador, O. Osorio,
F. Bolívar and F. Bastarrachea
Plasmid 3: 150-164, (1980).
25. Characterization of a site-specific restriction endonuclease
SphI from Streptomyces phaochromogenes.
L.Y. Fuchs, L. Covarrubias, L. Escalante, S. Sánchez
and F. Bolívar
Gene 10: 39-46, (1980).
26. Construction and characterization of new cloning vehicles;
deletions of pBR322 and pBR325.
X. Soberón, L. Covarrubias and F. Bolívar
In: Movilization and Reassembly of Genetic Information.
W.A. Scott, R. Werner, D.F. Joseph, J. Schultz, (eds). Academic
Press, New York. pp. 455, (1980).
27. Identification of a putative hypothalamic mRNA coding
for somatostatin and of its product in cell-free translation.
P. Joseph-Bravo, J.L. Charli, T. Sherman, H.W. Boyer, F.
Bolívar and J.F. Mc Kelvy
Biochem. Biophys. Res. Commun. 94: 1004-1012,(1980).
28. Cloning and physical mapping of the glnA gene
of Escherichia coli K -12.
A.A. Covarrubias, M. Rocha, F. Bolívar and F. Bastarrachea
Gene 11: 239-251,(1980).
29. Genetic characterization of mutations afecting glutamine
biosynthesis and its regulation in Escherichia coli
K -12.
F. Bastarrachea, S. Brom, A.A. Covarrubias, A. Osorio and
F. Bolívar
In: "Glutamine; metabolism, enzymology and regulation"
J. Mora, R. Palacios, (eds.) Academic Press-UNAM, México
D.F., pp. 107-120, (1980).
30. Isolation of Escherichia coli chromosomal DNA
regions carrying glnA, gdh and gltB structural
genes.
A.A. Covarrubias, R. Sánchez-Pescador, E. Lozoya,
F. Bastarrachea and F. Bolívar
In: "Glutamine; metabolism, enzymology and regulation"
J. Mora, R. Palacios, (eds.) Academic Press-UNAM, México
D.F., New York, pp. 123-137, (1980).
31. Tight linkage of genes that encode the two glutamate
synthase subunits of Escherichia coli K -12.
E. Lozoya, R. Sánchez-Pescador, A. Covarrubias, I.
Vichido and F. Bolívar
J. Bacteriol. 144: 616-621, (1980).
32. Construction and characterization of new cloning vehicles.
IV Deletion derivatives of pBR322 and pBR325.
X. Soberón, L. Covarrubias and F. Bolívar
Gene 9: 287-305, (1980).
33. Synthesis, cloning and expression of hormone genes in
Escherichia coli.
A. Riggs, K. Itakura, R. Crea, T. Hirose, A. Kraszewski,
D. Goeddel, D. Kleid, D. G. Yansura, F. Bolívar and
H. L. Heyneker
Recent Progress in Hormone Research 36: 261-274,
(1980).
34. Construction and characterization of new cloning vehicles.
V Mobilization and coding properties of pBR322 and several
deletion derivatives including pBR327 and pBR328.
L. Covarrubias, L. Cervantes, A. Covarrubias, X. Soberón.
I. Vichido, A. Blanco, Y.M. Kupersztoch-Pornoy and F. Bolívar
Gene: 13: 25-35, (1981).
35. Recombinant plasmids carrying the glutamate dehydrogenase
structural gene from Escherichia coli K -12.
R. Sánchez-Pescador, E. Sanvicente, F. Valle and
F. Bolívar
Gene 17: 1-8, (1982).
36. Construction and characterization of new cloning vehicles.
VI Plasmid pBR329, a new derivative of pBR328 lacking the
482- base-pair inverted duplication.
L. Covarrubias and F. Bolívar
Gene 17: 79-89, (1982).
37. Clonación molecular de DNA complementario a RNA
mensajero que codifica para preproinsulina de rata.
I. Vichido y F. Bolívar
Boletín de Estudios Médicos y Biológicos, 32: 13-29, (1982).
38. A new cloning vehicle in which the Cmr gene
is transcribed from a promoter within the Tcr gene.
L. Covarrubias and F. Bolívar
In: Promoters, Structure and Function.
R.L. Rodríguez and M.J. Chamberlin (eds.)
Praeger Scientific, New York, EUA. pp. 510-511, (1982).
39. Evidencias bioquímicas de la presencia del gene
estructural de la deshidrogenasa glutámica de Escherichia
coli K -12 en plásmidos recombinantes.
E. Sanvicente, R. Sánchez-Pescador, F. Valle y F.
Bolívar
Boletín de Estudios Médicos y Biológicos 32: 225-232, (1983).
40. The nucleotide sequence of the promoter and amino terminal
coding regions of the glutamate dehydrogenase structural
gene of Escherichia coli.
F. Valle, E. Sanvicente, P. Seeburg, A. Covarrubias, R.L.
Rodríguez and F. Bolívar
Gene 23: 199-209, (1983).
41. Structural organization of the genes that encode two
glutamate synthase subunits of Escherichia coli.
A. Garcíarrubio, E. Lozoya, A. Covarrubias and F.
Bolívar
Gene 26: 165-170, (1983).
42. Glucosa isomerasa: Sobreproducción de la enzima
por técnicas de ingeniería genética
molecular.
A. Farrés, F. Bolívar y S. Sánchez
En: "Biotecnología de Enzimas.
C. Huitrón (ed.)
Universidad Nacional Autónoma de México, pp.
258-269, (1983).
43. Complete nucleotide sequence of the glutamate dehydrogenase
gene from Escherichia coli K -12.
F. Valle, B. Becerril, E. Chen, P. Seeburg, H. Heyneker
and F. Bolívar
Gene 27: 193-199, (1984).
44. Construction and characterization of new cloning vehicles.
VII Construction of plasmid pBR327-PAR, a completely sequenced,
stable derivate of pBR327 containing the PAR locus of pSC101.
M. Zurita, F. Bolívar and X. Soberón
Gene 28: 119-122, (1984).
45. Clonación de un cDNA de interferon leucocitario
humano y su estrategia de producción en Escherichia
coli.
G. Oliver, P. Balbás, F. Valle, X. Soberón
y F. Bolívar
Revista Latinoamericana de Microbiología 27:
141-150, (1985).
46. Repetitive extragenic palindromic (REP) sequences in
the Escherichia coli gdhA gene.
B. Becerril, F. Valle, E. Merino, L. Riba and F. Bolívar
Gene 37: 52-62, (1985).
47. Identification and isolation of human insulin A y B
chains by HPLC.
O. Ladrón de Guevara, X. Alvarado, G. Estrada, S.
Antonio, F. Zamudio and F. Bolívar
J. of Chromatography 349: 91-98, (1985).
48. Influence of regions upstream the promoter for the primer
RNA on the copy number and stability of pBR327 derived plasmids.
H. Lomelí, M. Zurita, F. Bolívar and X. Soberón
In: Plasmids in Bacteria.
D.R. Helinski, S.N. Cohen, D.B. Clewell, D.A. Jackson and
A. Hollaender (eds.), Basic Life Science 30, pp.
866, (1985).
49. A common precursor for the two subunits of the penicillin
acylase from Escherichia coli ATCC 11105.
G. Oliver, F. Valle, F. Rosetti, M. Gómez-Pedrozo,
P. Santamaría, G. Gosset and F. Bolívar
Gene 40: 9-14, (1985).
50. Plasmid vector pBR322 and its special-purpose derivatives.
P. Balbás, X. Soberón, E. Merino, M. Zurita,
H. Lomelí, F. Valle, N. Flores y F. Bolívar
Gene 50: 1-38, (1986).
51. Characterization of the regulatory region of the Escherichia
coli penicillin acylase structural gene.
F. Valle, G. Gosset, B. Tenorio, G. Oliver, and F. Bolívar
Gene 50: 119-122, (1986).
52. A new expression vector for the production of fused
proteins in Escherichia coli.
N. Flores, R. de Anda, L. Güereca, N. Cruz, S. Antonio,
P. Balbás, F. Bolívar and F. Valle
Journal of Applied Microbiology and Biotechnology 25:
267-271, (1986).
53. An analytical HPLC method for the isolation and characterization
of insulin.
O. Ladrón de Guevara, I. Huerta, N. Cruz, L. Güereca,
S. Antonio and F. Bolívar
H. Kalaez and L.S. Ettre (eds.) Academia Kiado, Budapest
Chromatography 85: pp. 169-177, (1986).
54. Deletion of a repetitive extragenic palindromic (REP)
sequence downstream from the structural gene of Escherichia
coli glutamate dehydrogenase affects the stability of
its mRNA.
E. Merino, B. Becerril, F. Valle, and F. Bolívar
Gene 58: 305-309, (1987).
55. Determination of the nucleotide sequence for the glutamate
synthase structural genes of Escherichia coli K -12.
G. Oliver, G. Gosset, R. Sánchez-Pescador, E. Lozoya,
. L.M. Ku, N. Flores, B.Becerril, and F. Bolívar
Gene 60: 1-14, (1987).
56. The plasmid pBR322.
P. Balbás, X. Soberón, F. Bolívar and
R.L. Rodríguez
In: Vectors: A survey of molecular cloning vectors and their
uses Rodríguez and Denhart (eds.), Butterwworth Publishing
Company. Stoneham, Mass., USA, pp. 1-15, (1988).
Nota: Este libro ha sido traducido al ruso.
57. Overproduction of proteins by recombinant DNA: human
insulin.
P. Balbás, R. de Anda, N. Flores, X. Alvarado, N.
Cruz, F. Valle and F. Bolívar.
En: Cell Function and Disease; L. Cañedo, L. Bald
and L. Parker (eds.), Plenum Press, New York, USA, pp. 59-74,
(1988).
58. Cloning, sequencing and expression in ficoll-generated
minicells of an Escherichia coli heat-stable enterotoxin
gene.
H. Stieglitz, L. Cervantes, R. Robledo, R. Fonseca, L. Covarrubias,
F. Bolívar and Y.M. Kupersztoch
Plasmid 20: 42-53, (1988).
59. Plasmid pBR322: The multipurpose cloning vector.
F. Bolívar
Focus 10:, 61-64, (1988).
60. Identification of a functional promoter for the Escherichia
coli gdhA gene and its regulation.
L. Riba, B. Becerril, Servín-González, F.
Valle and F. Bolívar
Gene 71: 233-246, (1988).
61. Aminoacid sequence analysis of the glutamate synthase
enzyme from Escherichia coli K -12.
G. Gosset, E. Merino, F. Recillas, G. Oliver, B.Becerril
and F. Bolívar
Protein Sequence Data Analysis 1: 9-16, (1989).
62. The ribonucleoside diphosphate reductase gene (nrdA)
of Escherichia coli carries a repetitive extragenic
palindromic (REP) sequence in its 3' structural terminus.
E. Merino and F. Bolívar
Journal of Molecular Microbiology 3: 839-841, (1989).
63. Advances in the design and construction of expression
plasmid vectors.
P. Balbás and F. Bolívar
Recombinant DNA
In: Methods in Enzymology V.
D. Goeddel (Ed.) Academic Press, New York, 185: pp.
14-37, (1990).
64. Maximizing the expression of recombinant proteins in
E. coli.
E. Galindo, F. Bolívar and R. Quintero
Journal of Fermentation and Biotechnology 69: (3),
159-165, (1990).
65. Preparative purification of recombinant insulin B chain,
via HPLC.
N. Cruz, S. Antonio, R. de Anda, G. Gosset and F. Bolívar
J. Liquid of Chromatography 13: (8) 1517-1528, (1990).
66. Multipurpose tools in molecular biology.
F. Bolívar
Current Contents 47: 12, (1990).
67. The role of penicillin amidases in nature and in industry.
F. Valle, P. Balbás, E. Merino and F. Bolívar.
Trends in Biochemical Sciences 16: 36-40, (1991).
68. Production of human insulin.
G. Gosset, F. Bolívar and R. Quintero.
In: Biologicals from recombinant microorganisms and animal
cells.
M.D. White, S. Reuvery and A. Shafferman (eds).
VCH Publishers, Alemania, pp. 549-556, (1991).
69. New insights on the comma-less theory.
E. Merino, P. Balbás and F. Bolívar
Origins of Life and Evolution of the Biosphere 21:
251-254, (1992).
70. A general PCR-based method for single or combinatorial
oligonucleotide-directed mutagenesis on pUC/M13 vectors.
E. Merino, J. Osuna, F. Bolívar and X. Soberón
Biotechniques 12(4): 508-510, (1992).
71. Carbon regulation and the role in Nature of the E.
coli penicillin acylase (pac) gene.
E. Merino, P. Balbás, F. Recillas, B. Becerril, F.
Valle, and F. Bolívar
Molecular Microbiology 6(15): 2175-2182, (1992).
72. Preparative isolation of recombinant human insulin-A
chain by ion exchange chromatography.
N. Cruz, M. López, G. Estrada, X. Alvarado, R. de
Anda, P. Balbás, G. Gosset and F. Bolívar
J. of Liquid Chromatography 15(13): 2311-2324, (1992).
73. gltF a member of the gltBDF operon
of Escherichia coli is involved in nitrogen regulated
gene expression.
I. Castaño, N. Flores, F. Valle, A. Covarrubias and
F. Bolívar
Molecular Microbiology 6(18): 2733-2741, (1992).
74. Recovery of DNA from agarose gels stained with methylene
blue.
N. Flores, F. Valle, F. Bolívar and E. Merino
Biotechniques 13(2): 203-205, (1992).
75. Cloning and characterization of cDNAs that code for
Na+ -channel- blocking toxins of the scorpion
Centruroides noxiusHoffmann.
B.Becerril, A. Vázquez, C.García, M. Corona,
F. Bolívar and L.D. Possani
Gene 128: 165-171, (1993).
76. Recombinant protein production in cultures of an Escherichia
coli trp strain.
G. Gosset, R. de Anda, N. Cruz, A. Martínez, R. Quintero
y F. Bolívar
Applied Microbiology and Biotechnology 39(4/5): 541-546,
(1993).
77. Primary structure determination and cloning of the cDNA
encoding toxin 4 of the scorpion Centruroides noxiusHoffmann.
A. Vázquez, B. Becerril, B.M. Martin, F. Zamudio,
F. Bolívar y L.D. Possani
FEBS Letters 320(1): 43-46, (1993).
78. The genomic region encoding toxin gamma from the scorpion
Tityus serrulatuscontains an intron.
B. Becerril, M. Corona, M.C. Mejía, B.M. Martin,
S. Lucas, F. Bolívar y L.D. Possani
FEBS Letters 335(1): 6-8, (1993).
79. Plasmid pBRINT: a vector for chromosomal insertion of
cloned DNA.
P.Balbás, X.Alvarado, F.Bolívar y F.Valle
Gene 136: 211-213, (1993).
80. Kinetic study of penicillin acylase production by recombinant
E. coli in batch cultures.
O.T. Ramírez, R. Zamora, G. Espinosa, E. Merino,
F. Bolívar y R. Quintero
Process Biochemistry 29: 197-206, (1994).
81. Antisense overlapping open reading frames in genes from
bacteria to human.
E. Merino, P. Balbás, J.L. Puente y F. Bolívar
Nucleic Acids Research, 22: 1903-1908, (1994).
82. Strategies in the design of a penicillin acylase production
process.
A. Gómez, M. Rodríguez, S. Ospina, E. Merino,
F. Bolívar, O.T. Ramírez, R. Quintero y A.
López-Munguía
En: Advances in Bioprocess Engineering, E. Galindo y O.T.
Ramírez (eds.), Kluwer Academic Publishers, pp. 29-40,
(1994).
83. Production in Escherichia coli of a rat chimeric
proinsulin polypeptide carrying human A and B chains and
its preparative chromatography.
J. Olmos, M. Zamudio, G. Gosset, P. Balbás, F. Valle
y F. Bolívar
Journal of Biotechnology, 38: 89-96, (1994).
84. Cloning of genes encoding scorpion toxins: an interpretative
review.
B. Becerril, M. Corona, C. García, F. Bolívar
y L.D. Possani
Journal of Toxicology, 14(3): 339-357, (1995).
85. Cloning of the two pyruvate kinase isoenzyme structural
genes from Escherichia coli: the relative roles of
these enzymes in pyruvate biosynthesis.
E. Ponce, N. Flores, A.Martínez, F. Valle y F. Bolívar
Journal of Bacteriology, 177(19): 5719-5722, (1995).
86. A functional SpoA is required for maximal aprE expression
in Bacillus subtilis.
J. Olmos, V. Bolaños, S. Cansey, E. Ferrari, F. Bolívar
y F. Valle
FEBS Letters, 381: 29-31, (1996).
87. Pathway engineering for the production of aromatic compounds
in Escherichia coli.
N. Flores, J. Xiao, A. Berry, F. Bolívar y F. Valle
Nature Biotechnology, 14(5): 620-623, (1996).
88. A pBRINT family of plasmids for integration of cloned
DNA into the Escherichia coli chromosome.
P. Balbás, M. Alexeyev, I. Shokolenko, F. Bolívar
y F. Valle
Gene, 172: 65-69, (1996).
89. Basic and applied aspects of metabolic diversity: the
phosphoenolpyruvate node.
F. Valle, E. Muñóz, E. Ponce, N. Flores y
F. Bolívar
Journal of Industrial Microbiology, 17: 458-462,
(1996).
90. Effects of the sinR and degU32(Hy)
mutations on the regulation of the aprE gene in
Bacillus subtilis.
J. Olmos, R. de Anda, E. Ferrari, F. Bolívar y F.
Valle
Molecular and General Genetics, 253: 562-567, (1997).
91. Stimulation of glucose catabolism through the pentose
pathway by the absense of the two pyruvate kinase isoenzymes
in E. coli.
E. Ponce, A. Martínez, F. Bolívar y F. Valle
Biotechnology and Bioengineering, 58: 292-295, (1998).
92. Molecular cloning by plasmid vectors.
P. Balbás y F. Bolívar
En: Recombinant DNA Principles and Methodologies; J.J. Greene
y V.B. Rao, (eds.), Marcel Dekker, Inc., New York, NY, pp.
383-411, (1998).
93. pBRINT-Ts: a plasmid family with a temperature-sensitive
replicon, designed for chromosomal integration into the
lacZ gene of Escherichia coli.
S. LeBorgne, B. Palmeros, F. Valle, F. Bolívar y
G. Gosset
Gene, 223: 213-219, (1998).
94. Relationship between codon usage and sequence-dependent
curvature of genomes.
R. Jáuregui, F. O'Reilly, F. Bolívar y E.
Merino
Microbial & Comparative Genomics, 3(4): 243-253, (1998)
95. Kinetic characterization in batch and continuous
culture of Escherichia coli mutants affected in
phosphoenolpyruvate metabolism: differences in acetic
acid production.
R. Sigüenza, N. Flores, G. Hernández, A. Martínez,
F. Bolívar y F. Valle
World Journal of Microbiology and Biotechnology, 15:
587-592, (1999).
96. Characterization of the 5' subtilisin (aprE)
regulatory region from Bacillus subtilis.
J. Jan, F. Valle, F. Bolívar y E. Merino
FEMS in Microbiology Letters, 183: 9-15, (2000).
97. Engineering the Escherichia coli outer membrane
protein OmpC for metal bioadsorption.
N. Cruz, S. LeBorgne, G. Hernández-Chávez,
G. Gosset, F. Valle y F. Bolívar
Biotechnology Letters, 22: 623-629, (2000).
98. A family of removable cassettes designed to obtain
antibiotic-resistance-free genomic modifications of Escherichia
coli and other bacteria.
B. Palmeros, J. Wild, W. Szybalski, S. Le Borgne, G. Hernández-Chávez,
G. Gosset, F. Valle y F. Bolívar
Gene, 247: 255-264, (2000).
99. Relationship between whole proteome aminoacid composition
and static DNA curvature.
R. Jáuregui, F. Bolívar and E. Merino
Microbial and Comparative Genomics, 5(1): 7-15,
(2000).
100. Peptides and genes coding for scorpion toxins that
affect ion-channels.
L.D. Possani, E. Merino, M. Corona, F. Bolívar
and B. Becerril
Biochimie, 82(9): 861-869, (2001).
101. Improvement of the pBRINT-Ts plasmid family to obtain
marker-free chromosomal insertion of cloned DNA in Escherichia
coli.
S. LeBorgne, B. Palmeros, F. Bolívar and G. Gosset
Biotechniques, 30: 252-256, (2001).
102. Determination of 3-Deoxy-D-Arabino-Heptulosonate
7-Phosphate Productivity and Yield from Glucose in Escherichia
coli Devoid of the Glucose Phosphotransferase Transport
System.
José Luis Báez, Francisco Bolívar
and Guillermo Gosset
Biotechnology and Bioengineering, 73(6): 530-535,
(2001).
103. Construction of protein overproducer strains in Bacillus
subtilis by an integrative approach.
J. Jan, F. Valle, F. Bolívar and E. Merino
Applied Microbiology and Biotechnology, 55: 69-75,
(2001).
104. Characterization of sugar mixtures utilization by
an Escherichia coli mutant devoid of the phosphotransferase
system.
V. Hernández, F. Valle, F. Bolívar and G.
Gosset
Applied Microbiology and Biotechnology, 57: 186-191,
(2001).
105. Analysis of carbon metabolism in Escherichia coli
strains with an inactive phosphotransferase system by
13C labelling and NMR spectroscopy.
S. Flores, G. Gosset, N. Flores, A.A. de Graaf and F.
Bolívar
Metabolic Engineering, 4: 124-137, (2002).
106. Expression of galP and glk in a
Escherichia coli PTS mutant restores glucose transport
and increases glycolytic flux to fermentation products.
V. Hernández-Montalvo, A. Martínez, G. Hernández-Chávez,
F. Bolívar, F. Valle
and G. Gosset
Biotechnology and Bioengineering, 83(6): 687-694,
(2003).
107. Back to basics: pBR322 and protein expression systems
in E. coli.
P. Balbás and F. Bolívar
In: Methods in Molecular Biology, Vol. 267: Recombinant
Gene Expression: Reviews and Protocols, Second Edition,
P. Balbás and A. Lorence (eds.), Humana Press Inc.
USA., pp. 77-90, (2004).
108. Plasmid vectors for marker-free chromosomal insertion
of genetic material in Escherichia coli.
S. Le Borgne, F. Bolívar and G. Gosset
In: Methods in Molecular Biology, Vol. 267: Recombinant
Gene Expression: Reviews and Protocols, Second Edition,
P. Balbás and A. Lorence (eds.), Humana Press Inc.
USA., pp. 135-143, (2004).
109. Characterization of bacterial diversity in Pulque,
a traditional mexican alcoholic fermented beverage, as
determined by 16S rDNA analysis.
A. Escalante, M.E. Rodríguez, A. Martínez,
A. López-Munguía, F. Bolívar and
G. Gosset
FEMS Microbiology Letters, 235: 273-279, (2004).
110. Growth-rate recovery of Escherichia coli cultures
carrying a multicopy plasmid, by engineering of the pentose-phosphate
pathway.
S. Flores-Chávez, R. De Anda-Herrera, G. Gosset-Lagarda
and F. Bolívar
Biotechnology and Bioengineering, 87(4): 485-494,
(2004).
111. Metabolic engineering and protein directed evolution
increase the yield of L-phenylalanine synthesized from
gucose in Escherichia coli.
J.L. Báez-Viveros, J. Osuna, G. Hernández-Chávez,
X. Soberón, F. Bolívar and G. Gosset
Biotechnology and Bioengineering, 87(4): 516-524,
(2004).
112. Role of pyruvate oxidase in Escherichia coli
strains lacking the phosphoenolpyruvate: carbohydrate
phosphotranferase system.
N. Flores, R. de Anda, S. Flores, A. Escalante, G. Hernández,
A. Martínez, O.T. Ramírez, G. Gosset y F.
Bolívar
J. Mol. Microbiol. Biotechnol. 8: 201-221, (2004).
113. Culture of Escherichia coli under dissolved
oxygen gradients simulated in a two-compartment scale-down
system: metabolic response and production of recombinant
protein.
E.A. Sandoval Basurto, G. Gosset, F. Bolívar and
O.T. Ramírez
Biotechnology and Bioengineering, 89(4): 453-463,
(2004).
114. Adaptation for fast growth on glucose by differential
expression of central carbon metabolism and gal
regulon genes in an Escherichia coli strain lacking
the phosphoenolpyruvate: carbohydrate phosphotransferase
system.
N. Flores, S. Flores, A. Escalante, R. de Anda, L. Leal,
R. Malpica, D. Georgellis, G. Gosset, and F. Bolívar
Metabolic Engineering, 7: 70-87, (2005).
115. Metabolic pathway engineering for microbial production
of aromatic amino acids.
M. I. Chávez, A. Martínez, F. Bolívar
and G. Gosset
Recent Advances in Food Science, 5: 11-20, (2005).
116. Ingeniería metabólica para incrementar
el flux y rendimiento de etanol en Escherichia coli
etanologénica.
G. Huerta-Beristain, J. Utrilla-Carreri, G. Hernández-Chávez,
F. Bolívar, G. Gosset y A. Martínez
Revista Mexicana de Ingeniería Química,
4: 25-36, (2005).
117. Nutrient scavenging stress response in an Escherichia
coli strain lacking the phosphoenolpyruvate: carbohydrate
phosphotransferase system, as explored by gene expression
profile.
S. Flores, N. Flores, R. De Anda, A. González,
A. Escalante, J.C. Sigala, G. Gosset and F. Bolívar
J. Mol. Microbiol. Biotechnol., 10: 51-63, (2005).
118. Transcriptional and metabolic response of recombinant
Escherichia coli to spatial dissolved oxygen tension
gradients simulated in a scale-down system.
A.R. Lara, L. Leal, N. Flores, G. Gosset, F. Bolívar
and O.T. Ramírez
Biotechnology and Bioengineering, 93(2): 372-385,
(2006).
119. Expression of the melA gene from Rhizobium
etli CFN42 in Escherichia coli and characterization
of the enconded tyrosinase.
N. Cabrera-Valladares, A. Martínez, S. Piñero,
V. H. Lagunas-Muñoz, R. Tinoco, R. De Anda, R.
Vázquez-Duhalt, F. Bolívar and G. Gosset
Enzyme and Microbial Technology, 38: 772-779, (2006).
120. Replacement of the glucose phosphotransferase transport
system by galactose permease reduces acetate accumulation
and improves process performance of Escherichia coli
for recombinant protein production without impairment
of growth rate.
R. De Anda, A.R. Lara, V. Hernández, V. Hernández-Montalvo,
G. Gosset, F. Bolívar, and O.T. Ramírez
Metabolic Engineering, 8: 281-290, (2006).
121. Engineering Escherichia coli to improve culture
performance and reduce formation of by-products during
recombinant protein production under transient intermittent
anaerobic conditions.
A.R. Lara, C. Vázquez-Limón, G. Gosset,
F. Bolívar, A. López-Munguía and
O.T. Ramírez
Biotechnology and Bioengineering, 94(6): 1165-1175, (2006).
122. Optimum melanin production using recombinant Escherichia
coli.
V.H. Lagunas-Muñoz, N. Cabrera-Valladares, F. Bolívar,
G. Gosset and A. Martínez
Journal of Applied Microbiology, 101: 1002-1008,
(2006).
123. Metabolic engineering of Bacillus subtilis
for ethanol production: lactate dehydrogenase plays a
key role in fermentative metabolism.
S. Romero, E. Merino, F. Bolívar, G. Gosset, and
A. Martínez
Applied and Environmental Microbiology, 73(16):
5190-5198, (2007).
124. Tyrosinase from Rhizobium etli is involved
in nodulation efficiency and symbiosis-associated stress
resistance.
S. Piñero, J. Rivera, D. Romero, M.A. Cevallos,
A. Martínez, F. Bolívar and G. Gosset
Journal of Molecular Microbiology and Biotechnology, 13
35-44, (2007).
125. Growth recovery on glucose under aerobic conditions
of an Escherichia coli strain carrying a phosphoenolpyruvate:
carbohydrate phosphotransferase system deletion by inactivating
arcA and overexpressing the genes coding for glucokinase
and galatose permease.
N. Flores, L. Leal, J. Sigala, R. de Anda, A. Escalante,
O.T. Ramírez, G. Gosset and F. Bolívar,
13: 105-116, (2007).
126. The phosphotransferase system-dependent sucrose utilization
regulon enteropathogenic Escherichia coli strains
is located in a variable chromosomal region containing
iap sequences.
L.G. Treviño-Quintanilla, A. Escalante, A.D. Caro,
A. Martínez, R. González, J.L. Puente, F.
Bolívar and G. Gosset
Journal of Molecular Microbiology and Biotechnology, 13:
117-125, (2007).
127. Metabolic transcription analysis of engineered Escherichia
coli strains that overproduce L-phenylalanine.
J.L. Baez-Viveros, N. Flores, K. Juarez, P. Castillo-Espana,
F. Bolivar and G. Gosset
Microbial Cell Factories, 6: 30, (2007).
128. New insights on the role of the sigma factor RpoS
as revealed in Escherichia coli strains lacking
the phosphoenolpyruvate: carbohydrate phosphotransferase
system.
N. Flores, A. Escalante, R. de Anda, J.L. Báez,
E. Merino, B. Franco, D. Georgellis, G. Gosset, and F.
Bolívar
Journal of Molecular Microbiology and Biotechnology, 14:
176-192, (2008).
129. Utility of an Escherichia coli strain engineered
in the substrate uptake system for improved culture performance
at high glucose and cell concentrations: an alternative
to fed-batch cultures.
Alvaro R. Lara, Luis Caspeta, Guillermo Gosset, Francisco
Bolívar, and Octavio T.
Ramírez
Biotechnology and Bioengineering, 99(4): 893-901,
(2008).
130. Coutilization of glucose and glycerol enhances the
production of aromatic compounds in an Escherichia
coli strain lacking the phosphoenolpyruvate: carbohydrate
phosphotransferase system.
K. Martínez, R. de Anda, G. Hernández, A.
Escalante, G. Gosset, O. T.Ramírez, and F. Bolívar
Microbial Cell Factories, 7: 1, (2008).
131. Specific ethanol production rate in ethanologenic
Escherichia coli strain KO11 is limited by pyruvate
decarboxylase.
G. Huerta-Beristain, J. Utrilla, G. Hernández-Chávez,
F. Bolívar, G. Gosset, and A. Martínez
Journal of Molecular Biology and Biotechnology, 15:
55-64, (2008).
132. Analysis of bacterial community during the fermentation
of pulque, a traditional Mexican alcoholic beverage,
using a polyphasic approach.
A. Escalante, M. Giles-Gómez, G. Hernández,
M.S. Córdova-Aguilar, A. López-Munguía,
G. Gosset and F. Bolívar
International Journal of Food Microbiology, 124:
126-134, (2008).
133. Metabolic regulation analysis of an ethanologenic
Escherichia coli strain based on RT-PCR and enzymatic
activities.
M. Orencio-Trejo, N. Flores, A. Escalante, G. Hernández-Chávez,
F. Bolívar, G. Gosset and A. Martínez
Biotechnology for Biofuels, 1:8 1-13, (2008).
134. Metabolic engineering of Escherichia coli
for L-tyrosine production by the expression of the genes
coding for the chorismate mutase domain of the native
chorismate mutase-prephenate
dehydratase and a cyclohexadienyl dehydrogenase from Zymomonas
mobilis.
M.I. Chávez-Béjar, A.R. Lara, H. López,
G. Hernández-Chávez,
A. Martinez, O.T. Ramírez, F. Bolívar and
G. Gosset
Applied and Environmental Microbiology, 74: 3284-3290,
(2008).
135. The effect of heating rate on Escherichia coli
metabolism, physiological stress, transcriptional response,
and production of temperature-induced recombinant protein:
a scale-down study.
L. Caspeta, N. Flores, N.O. Pérez, F. Bolívar
and O.T. Ramírez
Biotechnology and Bioengineering, 102(2): 468-482, (2009).
136. Acetate metabolism in Escherichia coli strains
lacking phosphoenolpyruvate: carbohydrate phosphotransferase
system; evidence of carbon recycling strategies and futile
cycles.
J.C. Sigala, S. Flores, N. Flores, C. Aguilar, R. de Anda,
G. Gosset and F. Bolívar
Journal of Molecular Microbiology and Biotechnology, 16:
224-235, (2009).
137. Metabolic engineering for improving anthranilate
synthesis from glucose in Escherichia coli.
V. Balderas-Hernandez, A. Sabido, A. Martinez, F. Bolivar
and G. Gosset
Microbial Cell Factories, 8: 19, (2009).
138. Metabolic and transcriptional response of recombinant Escherichia coli to elevated dissolved carbon dioxide concentrations.
A. Báez, N. Flores, F. Bolívar and O.T. Ramírez
Biotechnology & Bioengineering, (en prensa).
139. Transcription analysis of central metabolism genes in Escherichia coli. Possible roles of sigma38 in their expression, as a response to carbon limitation.
L. Olvera, A. Mendoza-Vargas, N. Flores, M. Olvera, J.C. Sigala, G. Gosset, E. Morett and F. Bolívar
PLoS ONE, 4(10): e7466. Doi:10.1371/journal.pone.0007466, (2009).
140. Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system.
A. Escalante, R. Calderón, A. Valdivia, R. de Anda, G. Hernández, O.T. Ramírez, G. Gosset and F. Bolívar
Microbial Cell Factories, 9: 21, (2010).
141. Adaptive evolution of Escherichia coli inactivated in the phosphotransferase system operon improves co-utilization of xylose and glucose under anaerobic conditions.
V. E. Balderas-Hernández, V. Hernández-Montalvo, F. Bolívar, G. Gosset and A. Martínez Applied Biochemistry and Biotechnology, 163: 485-496, (2011).
142. Metabolic engineering of Escherichia coli for improving L-3, 4-dihydroxyphenylalanine (L-DOPA) synthesis from glucose.
A.J. Muñoz, G. Hernández-Chávez, R. de Anda, A. Martínez, F. Bolívar and G. Gosset Journal of Industrial Microbiology and Biotechnology, 38(11): 1845-1852, (2011).
143. Simulation of dissolved CO2, gradients in a scale-down system: a metabolic and transcriptional study of recombinant Escherichia coli.
A. Báez, N. Flores, F. Bolívar and O.T. Ramírez
Biotechnology Journal, 6(8): 959-967, (2011) published online: DOI: 10.1002/biot.201000407
144. New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol.
K. Martínez-Gómez, N. Flores, H.M. Castañeda, G. Martínez-Batallar, G. Hernández-Chávez, O.T. Ramírez, G. Gosset, S. Encarnación, and F. Bolívar
Microbial Cell Factories 11: 46, (2012).
145. New insights on transcriptional responses of genes involved in carbon central metabolism, respiration and fermentation to low ATP levels inEscherichia coli.
S. Soria, R. De Anda, N. Flores, S. Romero-García, G. Gosset, F. Bolívar, and J.L. Báez-Viveros
Journal of Basic Microbiology 52: 1-16, (2012).
146. Biotechnological production of L-tyrosine and derived compounds.
M.I. Chávez-Béjar, J.L. Báez-Viveros, A. Martínez, F. Bolívar and G. Gosset Process Biochemistry 47: 1017-1026, (2012).
147. Current knowledge of the Escherichia coli phosphoenolpyruvate-carbohydrate phosphotransferase system: peculiarities of regulation and impact on growth and product formation.
A. Escalante, A. Salinas Cervantes, G. Gosset and F. Bolívar Applied Microbiology and Biotechnology 94: 1483-1494, (2012).
148. Laboratory metabolic evolution improves acetate tolerance and growth on acetate of ethanologenic Escherichia coli under non-aerated conditions in glucose-mineral medium.
M.T. Fernández-Sandoval, G. Huerta-Beristain, B. Trujillo-Martinez, P. Bustos, V. González, F. Bolivar, G. Gosset and A. Martínez
Applied Microbiology and Biotechnology DOI 10.1007/s00253-012-4177 -y (2012).
149. Physiologic consequences of gluose transport and phosphoenolyruvate node modifications in Bacillus subtilis 168.
N. Cabrera-Valladares, L.M. Martínez, N. Flores, G. Hernández-Chávez, A. Martínez, F. Bolívar, and G. Gosset
Journal of Molecular Microbiology and Biotechnology DOI 10.1159/000339973 (2012).
150. Genetic changes during a laboratory adaptive evolution process that allowed fast growth in glucose to an Escherichia coli strain lacking the major glucose transport system.
G. Aguilar, A. Escalante, N. Flores, R. De Anda, F. Riveros-McKay, G. Gosset, E. Morett, and F. Bolívar
BMC Genomics 13: 385 doi: 10.1186/1471-2164-13-385 (2012).
151. Consequences of phosphoenolpyruvate:sugar phosphotransferase system and pyruvate kinase isozymes inactivation in central carbon metabolism flux distribution in Escherichia coli.
E. Meza, J. Becker, F. Bolívar, G. Gosset, and C. Wittmann
Microbial Cell Factories 11(1): 127, (2012).
152. A novel plasmid vector designed for chromosomal gene integration and expression: Use for developing a genetically stable Escherichia coli melanin production strain.
A. Sabido, L.M. Martínez, R. De Anda, A. Martínez, F. Bolívar and G. Gosset
Plasmid 69: 16-23, (2013).
153. Enhancing thermo-induced recombinant protein production in Escherichia coli by
temperature oscillations and post-induction nutrient feeding strategies.
L. Caspeta, A.R. Lara, N.O. Pérez, N. Flores, F. Bolívar and O.T. Ramírez
Journal of Biotechnology 167: 47-55, (2013).
154. Modification of glucose import capacity in Escherichia coli: physiologic onsequences and
utility for improving DNA vaccine production.
L.G. Fuentes, A.R. Lara, L.M. Martínez, O.T. Ramírez, A. Martínez, F. Bolívar and G.
Gosset
Microbial Cell Factories 12: 42, (2013).
155. Constitutive expression of selected genes from the pentose phosphate and aromatic
pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia
coli strain lacking PTS and pykF.
A. Rodríguez, J.A. Martínez, J.L. Báez-Viveros, N. Flores, G. Hernández-Chávez, O.T.
Ramírez, G. Gosset, and F. Bolívar
Microbial Cell Factories, 12: 86, (2013).
156. Efecto de la clonación del gen zwf sobre la producción de Shikimato en la cepa de
Escherichia coli PB12.SA22.
S. Carmona, F. Bolívar, and A. Escalante
BioTecnología, 17(3): 66-86, (2013).
157. Improving poly-3-hydroxybutyrate production in Escherichia coli by combining the increase
in the NADPH pool and acetyl-CoA availability.
S. Centeno-Leija, G Huerta-Beristain, M. Giles-Gómez, F. Bolívar, G. Gosset, and A.
Martínez
Antonie van Leeuwenhoek, 105(4): 687-696, (2014). DOI 10.1007/s10482-014-0124-5
158. Physiological and transcriptional characterization of Escherichia coli strains lacking
interconversion of phosphoenolpyruvate and pyruvate when glucose and acetate are
coutilized.
A. Sabido, J.C. Sigala, G. Hernández-Chávez, N. Flores, G. Gosset, and F. Bolívar
Biotechnology and Bioengineering DOI: 10.1002/bit.25177, (2014).
159. Inactivation of pyruvate kinase or the phosphoenolpyruvate:sugar phosphotransferase
system increases shikimic and dehydroshikimic acid yields from glucose in Bacillus subtilis.
C. Licona-Cassani, A. Lara, N. Cabrera-Valladares, A. Escalante, G. Hernández-Chávez,
A. Martínez, F. Bolívar, and G. Gosset
Journal of Molecular Microbiology and Biotechnology, 24: 37-45, (2014).
DOI: 10.1159/000355264
160. Global transcriptomic analysis of an engineered Escherichia coli strain lacking the
phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid
production in rich culture medium.
L. Cortés-Tolalpa, R.M. Gutiérrez-Ríos, L.M. Martínez, R. de Anda-Herrera, G. Gosset, F.
Bolívar, and A. Escalante
Microbial Cell Factories, 13: 28 DOI:10.1186/1475-2859-13-28, (2014). 161. Current perspectives on applications of shikimic and aminoshikimic acids in pharmaceutical
chemistry.
D.C. Díaz Quiroz, S.B. Carmona, F. Bolívar, and A. Escalante
Research and Reports in Medicinal Chemistry
4: 35.46, (2014). DOI: 2147/EEMC.S46560
162. Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds.
A. Rodríguez, J.A. Martínez, N. Flores, A. Escalante, G. Gosset, and F. Bolívar
Microbial Cell Factories, 13: 126, (2014). DOI: 10.1186/s12934-014-0126-z
163. Cathecol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 12-dioxigenase from Pseudomonas aeruginosa PA01.
V.E. Balderas-Hernández, L.G. Treviño-Quintanilla, G. Hernández-Chávez, A. Martínez, F. Bolívar, and G. Gosset
Microbial Cell Factories, 13: 16, (2014). DOI: 10.1186/S12934-014-0136-x
164. Screening and characterization of extracellular polysaccharides produced by Leuconostoc kimchii isolated from traditional fermented pulque beverage.
I. Torres-Rodríguez, M.E. Rodríguez-Alegría, A. Miranda-Molina, M. Giles-Gómez, R. Conca Morales, A. López-Munguía, F. Bolívar, and A. Escalante
SpringerPlus, 3: 583, (2014). DOI: 10.1186/2193-1801-3-583
165. Draft genome sequence of Leuconostoc mesenteroides P45 isolated from pulque, a traditional Mexican alcoholic fermented beverage.
F. Riveros-Mckay, I. Campos, M. Giles-Gómez, F. Bolívar, and A. Escalante
Genome Announcements, 2(6): 1-2, (2014). DOI: 10.1128/genomeA.01130-14
166. Production of cinnamic and p-hydroxycinnamic acid from sugar mixtures with engineered Escherichia coli.
A. Vargas-Tah, L. Martínez, G. Hernández-Chávez, M. Rocha, A. Martínez, F. Bolívar, and G. Gosset
Microbial Cell Factories, 14: 6, (2015). DOI: 10.1186/s12934-014-0185-1
167. Shikimic acid production in Escherichia coli: from classic metabolic engineering strategies to omics applied to improve its production.
J.A. Martínez, F. Bolívar, and A. Escalante
Frontiers in Bionengineering and Biotechnology 3: 145, (2015).
DOI: 10.3389/fbioe.2015.00145 |
